I have some older straws that were frozen using the french mixture. The post thaw motility was 85%. Even though nobody uses this mixture anymore, is it considered just as viable as EDTA? It is not recommened that the 2 types of staws be used in one insemination, but would doing so ruin chances of a pregnancy?
I'm assuming you're referring to the INRA '82 extender? If so, then there is no reason why you shouldn't go ahead and use it.
Viability is going to be variable between stallions - it may work, it may not.
The problem with mixing extenders in a single insemination is if there is a dramatic difference in ingredients, that could lead to issues with matters such as osmolarity. My preference would be to not mix. Depending upon how many straws you have and require for a single insemination dose, what about using only a single extender per insemination and deep horn insemination techniques?
I would need some tutoring to attempt the deep horn insemination. Perhaps my primary care vet could help me there. She has been very helpful because neither she, nor the other vets here want to do breedings anymore. One way to work around this was to use the P&E this year. Thank you for that information! The 2 mares came into heat and ovulated right on schedule! I did one with frozen, one with fresh. Now the wait.
Typically, deep horn insemination - as it requires manipulation per rectum - is performed by a veterinarian. If you are familiar with rectal palpation you will be able to do it with little trouble, but if you are not, I would discourage you from attempting without correct training.
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