There seems to be alot of different opinions about breeding with frozen.Some say to use half a dose just before ovulation and half just after. Some say a full dose just before and another full dose just after.Some say to use one dose just after ovulation.Seems alot of people use drugs. What is the best way and do you really need to use drugs?
Frank Berg Posted From: 126.96.36.199
Posted on Saturday, July 26, 2003 - 12:51 pm:
Splitting a dose is not a very good idea unless the dose is known to be much larger than required. You need about 200-300 million good swimmers to make it work, typical from a pool of about 800 million starters in a normal dose. Uterine fluid caused by the inflamatory response will make the second dose much less effective unless it is administered within a few hours or after there is sufficient time between each insemination for a resonable amount of uterine clearance. Keep in mind there is typically a much larger inflamatory response when using frozen semen. Also, considering the cost of the semen, it is much better for the mare and your pocketbook to do a few extra ultra-sounds, even just a few hours apart if required, to pinpoint the ovulation and ONE insemination. The goal is to be as successful as possible.
The research on time of insemination favours ovulation to a few hours prior. The best window is about 6 hours prior to 2-3 hours after. Outside of this window the results drop off significantly.
Drugs... help narrow down the window for insemination. Prostaglandin to lyse the corpus luteum and bring on the next heat earlier than normal by about 1 week. HCG is probably the most useful drug used with palpation and ultra-sound. HCG is given when a good sized follicle is present and encourages ovulation within 36hrs +- 12hrs (sometimes ovulation occurs even earlier). Using this protocol usually reduces costs associated with AI.
Other drugs are based on individual requirements. Oxitocin is most common post breeding and it does help with uterine clearance. Also, there are numerous vets using uterine flushes on all mares bred using frozen semen. Obviously your mare will have to be evaluated for a best course of action.
I would suggest some reading of "Breeding Management & Foal Development" or some similar books. As well, there a very good articles on this web site that are based on real research, not just word of mouth, myth and hearsay!
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