If you were in parts of the world where the inactivated virus vaccine "Artervac®" is used, then you would have to do a second shot after the first (a "booster"), but as I happen to know that you are in North America, where Arvac® is used, a single vaccination is all that is required to get adequate immunity for about a year. Note that you should vaccinate annually (or check titre levels are adequate).
Note too that you should still quarantine the mare for about 10-14 days (ideally) after breeding, as she may be shedding virus from the semen during that time, and although it will not pose a threat to her, it could to other unvaccinated horses.
Thanks Jos, Yes we are in western Canada, and yes she will be quarantined after breeding as well as when she gets the vaccine. Yipee!
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