Hi, I live in Northwest Indiana and had the vet out on 3-18-08 to do a uterian culture, cytology and biopsy on my mares. He said one mare had a soft follicle and looked like she could ovulate soon. The follicle was over 35 cm. The other mare had a 49 follicle and it wasn't as soft and he felt she would regress this one.
I just finished reading in a reproduction book that cultures and biospy taken during transition is likely to be inconclusive.
After spending a great deal of money on these tests, it worries me that I may have done these at the wrong time. Can anyone here help me with an answer. Is it likely I won't get an accurate reading?
The problem with a culture and cytology performed during transitional phase (or even winter anestrus come to that) is that the cervix has not been closed since shortly after the last ovulation, so the door to the incubator - so to speak - has been left slightly open for some time, thereby possibly allowing pathogenic access. If the mare has gone through transitional phase and had her first ovulation of the year, then the natural uterine immune response associated with elevated estrogen levels will have been likely to cleared any pathogenic presence in the uterus, or at least there is a good chance that it has. Hence, if you get a positive result, had you waited until after the first ovulation of the year, there is a good chance the mare would have cleared it herself.
As far as the biopsy is concerned, the tissue condition present during anestrus/transitional phase will not be the same as during the cycling portion of the year, but many path. labs will be able to give some indication of biopsy grade.
Looks like I've jumped the gun this year. Thank you so much for your response!
Jos, do you recommend redoing the cultures and biopsy after transitioning?
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