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When to do culture/cytology?

Equine-Reproduction.com Bulletin Board » Breeding Problem Mares - Volume 2 » When to do culture/cytology? « Previous Next »


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Cindy
Nursing Foal
Username: Cweber

Post Number: 13
Registered: 06-2005
Posted on Wednesday, March 03, 2010 - 08:52 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

I have a mare that I want to have a culture/cytology done on this year. All my past vets have done it early in their heat cycle and we have bred them if all looks well. I was going to a new vet this year (it is a big equine repro vet in our area that does embryo transfers) and they said they wanted to do it when she was not in heat but it essentially could be done anytime. Is it o.k. do have a culture/cytology done when not in heat? Due to a poor biopsy score we wanted to try a DMSO lavage so would like to do it early in the year to give her a chance to heal a cycle or so before breeding. Just wondering if she is not cycling if we can go ahead with the culture/cytology or are we best to wait?
 

Jos
Board Administrator
Username: Admin

Post Number: 2743
Registered: 10-1999
Posted on Thursday, March 04, 2010 - 06:05 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

It is recommended to ensure that the mare has started cycling for the year (i.e. is not still in winter anestrus). As far as whether to do the sampling during estrus or diestrus, there are valid arguments to be made (and often are! :-)) for sampling during either period. Although I personally prefer sampling during estrus, I would not not sample during diestrus if I had to for some reason. The only point to consider is that you would be well advised to follow up a diestrus sampling with a treatment of prostaglandin to bring the mare back into estrus in case there was a contaminant taken in at the time of sampling.
 

Cindy
Nursing Foal
Username: Cweber

Post Number: 14
Registered: 06-2005
Posted on Thursday, March 04, 2010 - 10:39 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

The vet said we could do her anytime but I guess I had better wait for atleast a month since she is in winter anestrus. The vet never said anything about that. Why can it not be done in winter anestrus? Have to do with the possibility of a contaminant being taken in? If we are going to follow the culture/cytology with a DMSO lavage, would that not "kill" a contaminent? Anyways, just curious. I am not in a huge time crunch so don't mind waiting for her to start cycling.
 

Dorthy Brown
Breeding Stock
Username: Dodib

Post Number: 419
Registered: 04-2006
Posted on Friday, March 05, 2010 - 08:15 am:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

What about during transition?? I had ours done this year and the mare was in transition. Vet never mentioned I should wait til she was in estrus
 

Jos
Board Administrator
Username: Admin

Post Number: 2744
Registered: 10-1999
Posted on Friday, March 05, 2010 - 04:34 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

Until the mare has had her first ovulation of the year, her cervix will not be closed. Consequently, one is likely to identify organisms in the uterus that are merely contaminants. Once the first ovulation has occurred, and the cervix has closed and the mare subsequently comes back into estrus, her own immune system should have had a chance to eliminate any pathogenic/organism presence. If after the first ovulation and the start of the second estrus they are still present, that is indicative of a need for treatment.

Although a DMSO lavage will probably take care of many organisms, in my book it would be better to get a true diagnosis prior to treatment, that way you have a "base line" to determine when/where the trouble started in the event that the DMSO does not clear any issues up.



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