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Serum Infusion

Equine-Reproduction.com Bulletin Board » Breeding Problem Mares - Volume 2 » Serum Infusion « Previous Next »


Author Message
 

cathy Cook
Breeding Stock
Username: Razmacat

Post Number: 244
Registered: 08-2005
Posted on Sunday, May 27, 2007 - 09:19 am:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

Jos, a few years ago I had some luck infusing mares post breeding with their serum. I would like to do it again this year for my end of season problem mares. How much blood needs to be bled out? How much serum do I need to infuse? Have you ever used dog plasma bags for serum collection?
 

Jos
Board Administrator
Username: Admin

Post Number: 1332
Registered: 10-1999
Posted on Sunday, May 27, 2007 - 10:28 am:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

How much you need wil depend a little upon what separation technique you are going to use. If you are centrifuging then typically 150-180 ml of blood will be sufficient, but if you are allowing separation by settlement you will need more - probably 180-240 ml of blood. You should end up with around 40-50 ml of plasma.

I have not used dog plasma, and would be uncomfortable doing so in case of cross-species issues.
 

cathy Cook
Breeding Stock
Username: Razmacat

Post Number: 246
Registered: 08-2005
Posted on Sunday, May 27, 2007 - 11:18 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

No not the dog plasma just the bags used to collect it. I was thinking of bleeding the mare into the bag and letting it seperate there.
 

Jos
Board Administrator
Username: Admin

Post Number: 1335
Registered: 10-1999
Posted on Monday, May 28, 2007 - 05:15 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

That would probably work (I have not used them), but if you take 60 or 80 ml syringes, draw the blood into 3 of those and allow them to separate by sitting them vertically, that will also work. If you do use the dog collection equipment, make sure of what there is in it in the way of separation/non-clot media (or not) and that it is suitable and compatible.
 

cathy Cook
Breeding Stock
Username: Razmacat

Post Number: 247
Registered: 08-2005
Posted on Monday, May 28, 2007 - 06:40 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

Will do, thanks. I think I am just going to draw the blood, give it to my vet and let her lab centrifuge it.



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