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Culture / Cytology Results

Equine-Reproduction.com Bulletin Board » Breeding Problem Mares - Volume 2 » Culture / Cytology Results « Previous Next »


Author Message
 

Lbeth
Neonate
Username: Lbeth

Post Number: 3
Registered: 03-2006
Posted on Thursday, September 21, 2006 - 02:06 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

I recently had a full repro pre-purchase exam done on a mare.

Mares history: 6 year old. 2004 Bred maiden and caught with one shipment of semen, healthy foal delivered. 2005 Bred on EARLY foal heat, no resulting pregnancy. Fluid found during preg check, cultured and infection found. Treated with several lavages, then re-bred (but never re-cultured?!) two more times (so three in all) no resulting pregnancy.

The message I was left by the attending vet was that the mare had normal exterior conformation, normal vaginal exam, no cysts, fluid or abnormal edema found upon ultrasound. The culture and cytology results were both negative.

Upon receiving the cytology paperwork the notes read: "a few neutrophils are present" and "mild catarrhal/neutrophelic inflammation".

My question is should the presence of ANY neutrophils be suspect of a problem, or are some levels considered "acceptable" or could be found in "clean/healthy" mares?

Second question: The culture was performed while this mare was NOT cycling. I assumed cultures should be done when the mare is in heat for the most accurate results?

My vet has recommended re-culturing and new cytology when this mare is cycling.

Any thoughts?
 

Jos
Board Administrator
Username: Admin

Post Number: 1026
Registered: 10-1999
Posted on Thursday, September 21, 2006 - 08:27 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

The presence of neutrophils and whether they are "normal" or not depends actually, in part, upon whether the mare was in estrus ("heat") when the sample was taken. If the swab was taken during estrus, as the cervix was relaxed (thereby perhaps allowing some pathogens into the uterus that results in neutrophil presence), a few neutrophils would not be a major cause for concern. As this sample was apparently taken during diestrus, there really should not have been any neutrophils. At that time, the cervix is closed and therefore any inflammatory cell presence indicates that there was something in the uterus that probably shouldn't have been there.

In view of the mares history, I would definitely have another culture and cytology performed, possibly also requesting that the culture evaluate for yeast/fungal presence as well.
 

Lbeth
Neonate
Username: Lbeth

Post Number: 4
Registered: 03-2006
Posted on Friday, September 22, 2006 - 11:18 am:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

VERY helpful response, exactly what I needed to know.

I will follow up with another culture and cytology.

Thank you very much!



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