I have been using a hemocytometer to monitor my stallion's semen everytime he is collected. I do the dilution 1:9 then 1:9 of the first mixture. Is it possible to use a pipette pump (10ul or 100ul?) and do just 1 dilution, if so how?
One could use the pipette pump, but in my experience it is easier to do the 1:9, 1:9 than try for a 1:99 sample.
You can also do your first dilution in warmed semen extender, which will reduce the concentration to a point where it will be easier to take a sample for motility evaluation. Then do your second sample in the formalin solution.
We use insulin syringes for measuring our small volumes. Cheap and easy...
Please note that opinions, product information, advice or suggestions posted on this bulletin board are not necessarily those of the management at Equine-Reproduction.com nor does the maintenance of the post position indicate an implicit or any endorsement of that information, opinion or product.
Further, although we have the greatest respect for the posters offering assistance here, you are advised to seek a consultation with your veterinarian prior to using information obtained from this board if it is of a veterinary nature.Proud to be sponsored and supported by: