MAIN PAGE
EQUINE REPRODUCTION ARTICLES
SHORT COURSES
OTHER SERVICES AVAILABLE FROM EQUINE-REPRODUCTION.COM
FROZEN SEMEN STALLIONS
CERTIFIED SEMEN FREEZING LOCATIONS
EQUINE REPRODUCTION SUPPLIES
EQUINE REPRODUCTION BOOKS
EQUINE REPRODUCTION LINKS
EQUINE REPRODUCTION E-MAIL LIST
EASILY CALCULATE THE CORRECT VOLUME OF SEMEN AND EXTENDER TO SHIP OR USE ON FARM!
EQUINE REPRODUCTION BULLETIN BOARD
SITE MAP OF EQUINE-REPRODUCTION.COM
CONTACT US

horse breeding
horse breeding
horse breeding
horse breeding
horse breeding
horse breeding
horse breeding
horse breeding
horse breeding
horse breeding
horse breeding
horse breeding
horse breeding
horse breeding
Go to the articles page
 
Equine-Reproduction.com Bulletin Board
 
Topics Page Topics Page Register for a new account Register Edit Profile Profile Log Out Log Out Help/Instructions Help    
New Posts New Posts Last 1|3|7 Days Search Search Tree View Tree View  
Posting is restricted to registered board members only to prevent spamming of the board. We regret the necessity of this action, but hope you will appreciate the importance of the integrity of the board. Registration is free and information provided during the process will not be submitted to third parties.

Question about Cytology/Culture

Equine-Reproduction.com Bulletin Board » Breeding Methods » Question about Cytology/Culture « Previous Next »


Author Message
 

hedgerow pony farm
Breeding Stock
Username: Lotsofponies

Post Number: 207
Registered: 03-2009
Posted on Sunday, July 17, 2011 - 11:16 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

How close to insemination can a cytology and culture be done? The reason I'm asking is a friend is breeding a mare AI, took it to the clinic, the mare was short cycled. She has remained at clinic so the vet can follow her cycle and she is suppose to be ready to breed tomorrow, a little sooner than they thought she would. The owner had asked the vet from day one to run a cytology and culture since the mare had been bred several times by frozen semen a couple of years ago and didn't get in foal. The vet said she was going to do it a day or so after insemination. So do you think the vet is doing the cytology/culture after insemination because she ran out of time since the folicle mature quicker than planned and didn't want to do it to close to insemination? What is to close to insemination to do a cytology/culture?
 

Jos
Board Administrator
Username: Admin

Post Number: 3359
Registered: 10-1999
Posted on Monday, July 18, 2011 - 12:45 am:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

The culture and cytology can be performed up to the moment of insemination, the only problem being that you would not get the results of the culture back in time. The cytology though, takes only about 5-10 minutes to prepare and be read and can be done stall-side or in the vet's clinic (we do them all the time), so you could have a "clean" reading on the cytology (if there are no inflammatory cells present) in plenty of time.

The old alarm bells are ringing though... <sigh>

What the **** is your vet talking about doing a culture and cytology AFTER breeding the mare for???? This is completely inappropriate for the following reasons:
  • When a mare is bred, she will mount an immune response against the sperm. This is completely normal, but a cytology taken a day or so after breeding will show MILLIONS of inflammatory cells (PMNs/neutrophils) as a result!!
  • There is a good chance that bacteria will be introduced during the breeding process, which will grow on culture and return a "positive" culture result - even though they are not normally present in the uterus and are therefore not a problem and are in fact almost certainly in the process of being taken care of by the millions of neutrophils present (see above) which is what those little fellows are there for!
  • Presuming the mare is close to ovulation at the time of breeding as she should be, a day or two later, the cervix may be about to close and it is not the right time to be sticking more things into the uterus from the outside, and possibly introducing new pathogens.
  • In the event that an organism is identified on culture, what the heck is the plan? As the mare will have ovulated - and indeed hopefully be pregnant - how does the vet plan on treating the alleged intra-uterine organism if one shows up????
I'm afraid this is one of those times when I am sitting here saying to myself "what the **** is this vet thinking?" and the answer is coming back to me loud and clear... "they aren't...". :-(

You may want to find another vet to do your reproductive work...



Please note that opinions, product information, advice or suggestions posted on this bulletin board are not necessarily those of the management at Equine-Reproduction.com nor does the maintenance of the post position indicate an implicit or any endorsement of that information, opinion or product.

Further, although we have the greatest respect for the posters offering assistance here, you are advised to seek a consultation with your veterinarian prior to using information obtained from this board if it is of a veterinary nature.

Proud to be sponsored and supported by:
IMV Technologies - makers of Equine AI Equipment
Equine A.I. Equipment Supplies
Universal Medical Systems Ultrasounds
For your Veterinary Ultrasounding Needs
Hamilton Research Inc - Home of the Equitainer
Hamilton Research Inc - Home of the Equitainer
Exodus Breeders Supply - Your one-stop shop for all your reproductive needs!
Exodus Breeders Supply
Har-Vet: An Industry Leader in Equine Veterinary Products
An Industry Leader in Equine Veterinary Products!
Reproduction Resources: Specializing in Artificial Breeding and Embryo Transfer Supplies
Specializing in Artificial Breeding and ET Supplies
BET Pharm: Your Compounding Pharmacy for Reproductive Needs!
Your Compounding Pharmacy for Reproductive Needs!
www.SemenTanks.com - Quality Tanks at Competitive Prices!
Quality Tanks at Competitive Prices!
J.L. Smith Co. - Safe, affordable breeding stocks!
Safe, affordable breeding stocks!
  International Veterinary Information Service
International Veterinary Information Service
 

MAIN PAGE | INFORMATIONAL ARTICLES | SHORTCOURSES | SERVICES
FROZEN STALLIONS | FREEZING LOCATIONS | SUPPLIES | BOOKS | LINKS
EQUINE REPRODUCTION E-MAIL LIST | SEMEN CALCULATOR | BULLETIN BOARD
SITEMAP | CONTACT US