How close to insemination can a cytology and culture be done? The reason I'm asking is a friend is breeding a mare AI, took it to the clinic, the mare was short cycled. She has remained at clinic so the vet can follow her cycle and she is suppose to be ready to breed tomorrow, a little sooner than they thought she would. The owner had asked the vet from day one to run a cytology and culture since the mare had been bred several times by frozen semen a couple of years ago and didn't get in foal. The vet said she was going to do it a day or so after insemination. So do you think the vet is doing the cytology/culture after insemination because she ran out of time since the folicle mature quicker than planned and didn't want to do it to close to insemination? What is to close to insemination to do a cytology/culture?
The culture and cytology can be performed up to the moment of insemination, the only problem being that you would not get the results of the culture back in time. The cytology though, takes only about 5-10 minutes to prepare and be read and can be done stall-side or in the vet's clinic (we do them all the time), so you could have a "clean" reading on the cytology (if there are no inflammatory cells present) in plenty of time.
The old alarm bells are ringing though... <sigh>
What the **** is your vet talking about doing a culture and cytology AFTER breeding the mare for???? This is completely inappropriate for the following reasons:
When a mare is bred, she will mount an immune response against the sperm. This is completely normal, but a cytology taken a day or so after breeding will show MILLIONS of inflammatory cells (PMNs/neutrophils) as a result!!
There is a good chance that bacteria will be introduced during the breeding process, which will grow on culture and return a "positive" culture result - even though they are not normally present in the uterus and are therefore not a problem and are in fact almost certainly in the process of being taken care of by the millions of neutrophils present (see above) which is what those little fellows are there for!
Presuming the mare is close to ovulation at the time of breeding as she should be, a day or two later, the cervix may be about to close and it is not the right time to be sticking more things into the uterus from the outside, and possibly introducing new pathogens.
In the event that an organism is identified on culture, what the heck is the plan? As the mare will have ovulated - and indeed hopefully be pregnant - how does the vet plan on treating the alleged intra-uterine organism if one shows up????
I'm afraid this is one of those times when I am sitting here saying to myself "what the **** is this vet thinking?" and the answer is coming back to me loud and clear... "they aren't...".
You may want to find another vet to do your reproductive work...
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