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Oxytocin article - questions about the protocol

Equine-Reproduction.com Bulletin Board » Breeding Methods » Oxytocin article - questions about the protocol « Previous Next »


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Jennifer (Unregistered Guest)
Unregistered guest
Posted From: 198.230.169.216
Posted on Monday, July 18, 2005 - 09:41 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

After the intra-uterine infusion of a broad spectrum antibiotic do you need to do another lavage with just saline for a final clean of the uterus? or do you just continue on with the oxytocin alone.

Does the antibiotic cause any issues for when the embryo enters the uterus on day 5/6?
 

Jos
Board Administrator
Username: Jos

Post Number: 10226
Registered: 10-1999
Posted on Monday, July 18, 2005 - 09:58 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

The antibiotic infusion is low-volume, and you follow it with oxytocin treatment only. No need for a lavage unless indicated (e.g. unresolved large volumes of intrauterine fluid).

We reserve lavages for large volumes of intrauterine fluid (>2cm depth) and tend to rely on oxytocin only in most cases, using antibiotics in only the more susceptible mares.

You should be using a non-irritating antibiotic. Unless indicated for some reason, stay away from the aminoglycocides (Gentamycin and Amikacin) which tend to be more irritating to the uterus. One that is broad spectrum and has been popular for quite a few years is "Timentin" (note that website is relative to human use, but gives the information about the product). As the antibiotic treatment takes place no further than 3 days after ovulation, there should be ample time for the uterus to "quieten down" following treatment and before the conceptus arrives - assuming a suitable antibiotic is used.
 

Jennifer (Unregistered Guest)
Unregistered guest
Posted From: 198.230.170.190
Posted on Thursday, July 21, 2005 - 11:52 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

We just bred another mare with frozen semen. We gave the first oxytocin shot 4 hours after breeding and when we ultrasounded her at 6 hours just before lavaging she had absolutely no fluid and her uterus looked great.

We decided to not lavage since there was no fluid or irritation. Our thinking was why add fluid when there isn't any.

Is this ok or should we always lavage regardless?

Thanks so much!
 

Jos
Board Administrator
Username: Jos

Post Number: 10233
Registered: 10-1999
Posted on Friday, July 22, 2005 - 12:00 am:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

As noted above, we reserve lavages for large volumes of intrauterine fluid (>2cm depth) and tend to rely on oxytocin only in most cases.

I would recommend repeated oxytocin use though - do not rely on the single dose even in the absence of fluid. You may not need the full 3½ day treatment of the seriously susceptible mare, but a couple more treatments at 6-hourly intervals will probably be beneficial.
 

Jennifer (Unregistered Guest)
Unregistered guest
Posted From: 198.230.170.88
Posted on Tuesday, July 26, 2005 - 11:15 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

Thanks so much!!!

One last question on this. Bred a new mare, 7 years old, who has been bred successfully with frozen numerous times. The AI breeding went great with no issues and the semen was really good but for the first time we saw a major reaction to the frozen semen post breeding in the uterus. We gave oxytocin 5 hours post breeding and then 7 hours post breeding we flushed with warm saline (horrible bloody return with a few particles) and gave excenel because of the reaction. We then flushed with just warm saline 12 hours after the first flush and there was a better return (slightly cloudy) with a few white flecks in the uterus. We checked 12 hours after this and the uterus looked better but as precaution we flushed again and left excenel in. The return on this flush was almost clear. We continued with the oxytocin regime of every 6 hours (except after adding the antibiotic) during this time.

Now we are 56 hours post breeding (we bred the mare within 4 hours of ovulation) and the uterus is looking a lot better - the horn is clear BUT the uterine body still has a small pocket of fluid 9mm. The article mentioned the cervix closes in 3 1/2 days or 84 hours post ovulation. We are noticing things tightening up. Does the cervix vary in the time it closes? or can we continue the oxytocin every 6 hours in hope of getting that fluid out in the last 28 hours?



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