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Pre breeding oxytocin

Equine-Reproduction.com Bulletin Board » Breeding Methods » Pre breeding oxytocin « Previous Next »


Author Message
 

Anonymous
 
Posted From: 65.138.1.230
Posted on Monday, July 11, 2005 - 06:19 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

How long do you wait to inseminate after giving oxytocin? Vet said 4 hours. Oxytocin protocal says there is uterine clearance in 15 to 30 minutes. One thread about urine pooling said 2 hours. CONFUSED.
 

Jos
Board Administrator
Username: Jos

Post Number: 10205
Registered: 10-1999
Posted on Monday, July 11, 2005 - 07:24 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

All of the above are sort of correct :-)

The half-life of oxytocin in the body is about 12 minutes, and 5 half-lives - pharmacologically speaking - represent elimination from the body. Hence, after an hour [pharmacologically speaking] there is a minimal circulating level of the drug remaining.... however one has to take into account individual animal and environmental variations, so one would generally consider that 2 hours would be a minimum time to give reasonable expectation of elimination, and to be safe, we generally will not breed earlier than 3 hours after giving oxytocin if we can avoid it (and definintely not 2 hours). 4 hours is playing it very safe.

With reference to the oxytocin protocol article - the drug is active and the body responding (with uterine contractions) in 15-30 minutes - but that does not represent the entire duration of that response period, but the elapse time to response.
 

Anonymous
 
Posted From: 65.138.1.230
Posted on Monday, July 11, 2005 - 07:39 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

Ok, Thanks.
Now, I gave her oxytocin and HcG at 5:00 pm.(she has a 37mm folicle on the right and a 42mm on the left)I'm going to inseminate her between 8:00 and 9:00. Is it then correct to inseminate again 24 hours later, (after the 5:00 injections) and again at the 40 hour mark?
 

Jos
Board Administrator
Username: Jos

Post Number: 10206
Registered: 10-1999
Posted on Monday, July 11, 2005 - 07:44 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

If you are following the timed insemination protocol, then the insemination times would be 24 and 40 hours after giving the hCG, so I'm a little hazy on why you are inseminating her so soon after giving the hCG...

And are you using fresh, cooled or frozen semen?
 

Anonymous
 
Posted From: 65.138.1.230
Posted on Monday, July 11, 2005 - 07:51 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

fresh. Partly because I have help available tonight and may not the next 2 days. Am I creating a bigger problem this way? I'll get help some how tomarrow.
 

Anonymous
 
Posted From: 65.138.1.230
Posted on Monday, July 11, 2005 - 07:53 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

And the vet told me to inseminate tonight because the semen would last for 48 hours. Is that correct?
 

Jos
Board Administrator
Username: Jos

Post Number: 10207
Registered: 10-1999
Posted on Monday, July 11, 2005 - 11:28 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

OK... that's starting to make more sense :-)

Assuming the stallion has known fertility and sperm longevity, there is no point in insemination tomorrow (24 hours after the first) if you inseminated tonight. Inseminate again 48 hours later if needed - your vet is correct in that it is viable for 48 hours assuming all qualities being equal (i.e. fertile stallion). The more insemination that take place, the more the uterus is irritated, and the more chance of introducing a contaminant to the uterus.
 

Anonymous
 
Posted From: 65.138.3.24
Posted on Tuesday, July 12, 2005 - 10:06 am:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

ok, well I know the stallion is fertile (we got his first colt this year), but I don't know about his longevity.
Ok, now you're going to think I'm a complete idiot...I inseminated her last night and when I pulled my hand out, I had a little bit of blood on my finger tips. this has never happened before. I'm assuming I probably scratched the cervix with the pipette. Do you think that is what happened and will the blood have any affect on the semen?
One more... My stallion had not been collected in a few days and he more than filled a collection bottle. We thought that was unusual for him. He's come real close to the top before, but never overflowed it like this time. Do you think some of it could have been the lubricant from the AV and if so, will that affect the semen. We inseminated the whole bottle. I've seen you and Kathy pour of the top of the bottle before but I don't know enough(as I'm sure you've gathered by now) to try that.



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