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How do you work a centrifuge?

Equine-Reproduction.com Bulletin Board » Miscellaneous and Suggestions for a New Topic Category » How do you work a centrifuge? « Previous Next »


Author Message
 

Marilyn Lemke - Dora due 7/31/08
Senior Stallion or Mare
Username: Marilyn_l

Post Number: 1055
Registered: 06-2007
Posted on Sunday, February 24, 2008 - 07:19 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

Okay, I did something really dumb, I bought a centrifuge to separate plasma from fresh blood and I don't know how to use it.

Can someone explain the process to me? I'm not sure if you put the vial in the metal tube or pour the blood in the metal tube. How do you balance it?

Also, how long do you centrifuge the blood to get the plasma?

If someone could explain the process to me, I would be very much indebted to them forever! :-)

Thank you very much for any help!!!!
Marilyn
 

Tracy Smith, Tali due 6/08
Breeding Stock
Username: Tracys

Post Number: 852
Registered: 08-2007
Posted on Monday, February 25, 2008 - 12:40 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

You put the tube of blood in the metal tube. Use another empty tube and put water in it and put it opposite of the blood to balance it, spin for 10 minutes and voila! You will have plasma
 

Marilyn Lemke - Dora due 7/31/08
Senior Stallion or Mare
Username: Marilyn_l

Post Number: 1059
Registered: 06-2007
Posted on Monday, February 25, 2008 - 01:42 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

Do you take the top off the tube of blood? How do you take the tube out of the centrifuge without mixing the blood and plasma? Because we tried to work the thing yesterday and tried to take the tube out of the centrifuge. It was kinda stuck because the top was on and it got mixed up. After 10 minutes it didn't seem to separate, but it probably was due to Mike trying to shake it out of the metal tube. lol
 

Marilyn Lemke - Dora due 7/31/08
Senior Stallion or Mare
Username: Marilyn_l

Post Number: 1061
Registered: 06-2007
Posted on Monday, February 25, 2008 - 01:53 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

I thought of another question. How much water do you add to the other tube? Do you have to weigh the blood tube and weigh the water tube to get the same weight, or can you put the same amount of water in the opposite tube as the blood?
 

Tracy Smith, Tali due 6/08
Breeding Stock
Username: Tracys

Post Number: 857
Registered: 08-2007
Posted on Monday, February 25, 2008 - 04:05 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

I've always used a "tiger top" tube that has a plasma seperator at the bottom so after spinning it moves up and stays between the plasma and the blood. You don't have to use this type of tube but just be careful removing the tube from the centrifuge (and yes, leave the top on or you will have blood spattered everywhere! :-) ) You are probably using too small of tubes, the top of the tube should be just over the metal part so you can grab the top and just pull out.

With the water I just put the same amount in as the tube of blood.
 

Marilyn Lemke - Dora due 7/31/08
Senior Stallion or Mare
Username: Marilyn_l

Post Number: 1063
Registered: 06-2007
Posted on Monday, February 25, 2008 - 04:39 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

Thank you Tracy, you are a sweetheart!!!! I'm indebted to you forever. :-)

I didn't know they came in different sizes. I think I've got it. Thank you again!
 

Tracy Smith, Tali due 6/08
Breeding Stock
Username: Tracys

Post Number: 858
Registered: 08-2007
Posted on Monday, February 25, 2008 - 06:06 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

You're welcome :-)



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