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What do you think of this concoction?

Equine-Reproduction.com Bulletin Board » Breeding Problem Mares - Volume 1 » What do you think of this concoction? « Previous Next »

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Donna (24.26.56.241)
Posted on Wednesday, June 12, 2002 - 12:42 am:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

A little history:
4 year old maiden mare. Bred three times last year. On second attempt had preg. lost at 21 days with fluid on uterus (had been injected w/ Oxy). Culture revealed severe yeast infection. Fluhed for 5 days with 3L saline... then bred.
Yeast gone... no pregnancy.

This year:
Bred once. No GO! Now on culture she has: 1) E. Coli 2)Staph (not Aureus) 3) Bacillus (non- pathogenic) 4) No beta hemolytic strep

Sensitive to Amikacin, Ticarcillin and a flouroquinolone I can't remember the name of...

She is in her first or second day of her heat and lasts 5 days. What can I do to attempt to breed her (and conceive!!) this cycle.

It was suggested that I lavage and flush her with: 500 ml (total) saline: with 10cc of B12, 1 cup of white vinager, and 16ml of Amikacin added. I am to do this for as many days as possible before breeding. After AI (fresh cooled extended with Amikacin) give HCG shot...Followed up with oxytocin 6 hours after insemination.

The "recommended" use is to INFUSE, not flush for three days. I have no idea about the other "stuff". I suppose the vinegar helps keep the ph balanced by increasing acidity? B12? No guesses.

ANy other suggestions??
 

Jos (142.177.105.186)
Posted on Wednesday, June 12, 2002 - 10:03 am:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

> Now on culture she has: 1) E. Coli 2)
> Staph (not Aureus) 3) Bacillus (non- pathogenic) 4) No beta
> hemolytic strep

What did the cytology show? If a cytology smear was not prepared in conjunction with the culture of the swab sample, then the results of the culture as far as showing pathogenic status of the uterus are WORTHLESS!!!! It is essential that if a "positive culture" result is seen that there be neutrophils also seen on the cytology smear - without those neutrophils present, it is most likely that the culture result is indicative of a contaminated sample! So - no cytology, no accuracy!

> It was suggested that I lavage and flush her with: 500 ml (total)
> saline: with 10cc of B12, 1 cup of white vinager, and 16ml of
> Amikacin. For as many days as possible before breeding. After AI
> (fresh cooled extended with Amikacin) give HCG shot...Followed up
> with oxytocin 6 hours after insemination.

I believe this is overkill and is likely to upset the uterus to the point where conception (or at least pregnancy maintenance) will be unlikely this cycle.

If it were my mare, I would infuse 3 times with an antibiotic to which the pathogen is sensitive, twice before breeding, once after. I might feel inclined to include some grapefruit seed extract in the infusion solution (2 drops) to dissuade possible superinfection, but where I am wanting to breed on this cycle, I might not.... My personal jury is out on this as I suspect that the grapefruit seed extract works by significantly changing the pH of the uterus, which I don't want to do if I'm about to breed/already have bred. I'd also use the oxytocin protocol post-breeding that's laid out on my web site and can be accessed by "clicking" here.

> I suppose the vinegar helps keep
> the ph balanced by increasing acidity? B12?

No - the vinegar would increase the acidity further. Amikacin is acidic and will require buffering with sodium bicarbonate prior to introduction to the uterus.
 

Donna (24.26.56.241)
Posted on Wednesday, June 12, 2002 - 10:57 am:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

Does the Amikacin Sulfate (designed for the uterus) have to buffered as well? If so how much Sodium Bicarbonate?

Would Ticarcillin be a better choice? Would it be less "caustic" to the uterus environment?

There is less fluid infused with the Ticarcillin... have you found this to be better... or worse?

Thank you in advance!



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