New here but this looks like the spot to post this. Our stallion is quite comfy with a phantom and has been used at clinics to teach newbys on the 'HOW TO' collect a stallion for breeding because he is easy. So am good there.
Question #1 is: we are collecting him here today ourselves for our own mares. So we will not be extending. What is the proper water temp for the AV and is there a tried and true technique to be sure it is not too tight or too loose??
Question #2 is: although we have a vet that will be here to prep the semen we collect today, I am curious what would be the standard procedure to do so and what amount of time are we looking at before we start loosing motility, quality, etc. In essence, how quickly do we need to be inseminating the mare with the fresh semen??
Q1: You should have the internal temperature of the AV between 45° and 54° C (113-130° F) as he likes it (some like it at the warmer end, some cooler). Note that this means that the water temperature going in will need to be hotter. As far as tightness, that too is a personal liking of the stallion - some tighter, some looser. You can estimate how tight or loose you have the AV relative to the stallion by comparing his penis to your arm, and inserting your (gloved) arm in the AV when lubricating it and adjusting the tightness as desired at that point.
Q2: Standard protocol is:
Take to warm (70°+ F) lab;
Remove small sample for evaluation;
Add equal volume of warm (body temperature) extender taken from the incubator;
Perform final dilutions as required following evaluation.
Decrease in motility and fertility will start immediately after ejaculation. With some stallions reduction in quality is rapid owing to toxic seminal plasma and/or temperature shock (particularly the latter in cold weather); with other stallions the semen may remain almost the same condition as following ejaculation for hours. You will find that everything is very much individual stallion-variable.
Incidentally, even though you are using the semen on-farm, the addition of a suitable antibiotic extender is always recommended - particularly if there is going to be a delay between collection and insemination.
Thank you for this information Jos. I had thought the water temp should be between 100-110F initially and is the main reason for asking.
And absolutely agree re: using extender even with on-farm inseminations however in this particular incidence we are not able as the extender has not arrived yet and the mares are ready
Our stallion typically (using extender of course) is quite motile for past the typical time frame so we feel good about that. My concern was just how quickly we need to be inseminating before the quality of the collection is poor.
I am going to assume that if the mares are in their stalls and ready to be inseminated as soon as the collection is complete, that once the semen is split accordingly we should be well within the time frame of using quality/viable semen.
We have previous (using extender) settled up to 3 mares with one collection so feel good about splitting it for just two.
Mares have went from being transitional to progressing much quicker than we all anticipated which has caused a bit of dismay. None the less, we move forward.
Thank you for your post.
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