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Latest frozen semen methods

Equine-Reproduction.com Bulletin Board » Frozen Semen » Latest frozen semen methods « Previous Next »


Author Message
 

dena sanders
Neonate
Username: Dbts

Post Number: 1
Registered: 06-2009
Posted on Wednesday, June 17, 2009 - 03:59 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

What procedures for inseminating using frozen semen has everyone been using with success? How do they compare to each other? For example: post ovulation, timed insemination protocol, deep horn, etc. And what is the latest, greatest, most popular method?
Thanks very much.
 

Jos
Board Administrator
Username: Admin

Post Number: 2501
Registered: 10-1999
Posted on Wednesday, June 17, 2009 - 11:30 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

This is rather a large topic!

In a nutshell:
  • Deep-horn insemination is the newest insemination technique, using a flexible pipette or a videoendoscope with a biopsy channel. Can be useful for low volume/low sperm number inseminations, but not necessary when dealing with good (fertile) semen;
  • The timed insemination protocol is described in the article on our site at that link;
  • Some research suggests little difference in pregnancy rates between insemination pre- or post-ovulation if within the 12 hours before to 6 hours after ovulation window. Other research suggests a marginal increase in pregnancy rates if insemination takes place before or both before and after ovulation (again within the same time frame window).
A lot will depend upon the number of doses of semen available, and the level of knowledge and competence of the inseminator.
 

Carol
Yearling
Username: Rodawn

Post Number: 98
Registered: 05-2009
Posted on Monday, December 14, 2009 - 01:12 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

Jos, how hard is it for a mare owner to do their own inseminations with frozen semen? Is there an article somewhere on your site that lists all of the equipment one would need to outfit a lab for this? I'm thinking of doing all of my own insemination in 2010 and so would like to find some intensive articles with all the in-depth nitty-gritty details and with pictures if possible (for us visual and hands-on learners).
 

Jos
Board Administrator
Username: Admin

Post Number: 2663
Registered: 10-1999
Posted on Monday, December 14, 2009 - 08:32 pm:   Edit Post Delete Post    Move Post (Moderator/Admin Only)

There are article on timed insemination, thawing frozen semen, and insemination all to be found in the articles section.

The issue is achieving insemination within the 12 hours before to 6 hours after ovulation that is required for optimum pregnancy rates. If you cannot achieve that time of insemination, you are not going to have good success. Palpation or ultrasound obviously make that timing achievable. Alternatively, check out the timed insemination protocol that is coupled with P&E usage, which will assist.



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