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How to freeze embryo - immediate freeze or cool down

Discuss the pros and protocols, and the cons and costs of embryo transfer.

Moderator: Jos

How to freeze embryo - immediate freeze or cool down

Postby horseofcorff » Mon Nov 26, 2012 9:13 am

I have seen references to freezing embryos (and my own vet confirms) that you just put the straw with the embryo/cryoprotectant media directly into liquid nitrogen, unlike freezing semen what requires a slow cool down. Recently I saw sites that recommend a slow cool down for shipping fresh embryos. So is the new consensus to slow cool the embryo in a similar manner to semen when freezing? Any studies or references out there?
horseofcorff
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Re: How to freeze embryo - immediate freeze or cool down

Postby Jos » Mon Nov 26, 2012 2:09 pm

You're not comparing apples to apples, but apples to pears here... :)

For vitrification of embryos, there is an instant freeze, but for cooled shipping of embryos an Equitainer or similar container is recommended, which has a passive cooling device within.

When freezing semen it can be taken from room temperature to -160°C almost immediately and still retain fertility (depending upon stallion and protocol), while successfully shipping cooled semen requires a cool-down rate of ~0.05°/ minute.
We're always happy to try and help, but don't forget to check the articles section
of the website too, which has a search engine to help you look for answers!
:)
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Re: How to freeze embryo - immediate freeze or cool down

Postby horseofcorff » Tue Nov 27, 2012 8:59 pm

Thanks.
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