MAIN PAGE
EQUINE REPRODUCTION ARTICLES
SHORT COURSES
ONE-ON-ONE TUITION
OTHER SERVICES AVAILABLE FROM EQUINE-REPRODUCTION.COM
SEMEN EXPORT SERVICES
STALLIONS AT EQUINE-REPRODUCTION.COM
FROZEN SEMEN STALLIONS
CERTIFIED SEMEN FREEZING LOCATIONS
EQUINE REPRODUCTION SUPPLIES
EQUINE REPRODUCTION BOOKS
EQUINE REPRODUCTION LINKS
EQUINE REPRODUCTION E-MAIL LIST
EASILY CALCULATE THE CORRECT VOLUME OF SEMEN AND EXTENDER TO SHIP OR USE ON FARM!
EQUINE REPRODUCTION BULLETIN BOARD
CONTACT US


If you "like" us please "recommend" us!



and then:
Join us on Facebook! Join us on Facebook!


Proudly sponsored by:

Hamilton Research Inc - Home of the Equitainer
Hamilton Research Inc
Home of the Equitainer


Home of the world-famous INRA '96 extender!
Home of the world-famous
INRA '96 extender!


Universal Medical Systems Ultrasounds
For your veterinary
ultrasounding needs


Har-Vet: An Industry Leader in Equine Veterinary Products
An Industry Leader in
Equine Veterinary Products!


BET Pharm: Your compounding pharmacy for reproductive needs!
Your compounding pharmacy for
reproductive needs!


www.SemenTanks.com - Quality Tanks at Competitive Prices!
Quality Tanks at
Competitive Prices!


J.L. Smith Co. - Safe, affordable breeding stocks!
Safe, affordable
breeding stocks!


Here Today. There Today.
Here Today. There Today.


Saving Foals from FPT
Saving Foals from FPT


Egg Tech - serving European breeder's needs!
Egg Tech - serving
European breeder's needs!


International Veterinary Information Service
International Veterinary
Information Service






Please note that this records only identifiable - not all - hits!

Single Layer Centrifugation (SLC)

Discuss tips and tricks used in live cover and AI situations.

Moderator: Jos

Single Layer Centrifugation (SLC)

Postby lotsofponies » Thu Jan 31, 2013 1:07 pm

Was reading an article about the benifits of Single Layer Centrifugation (SLC)
The benifits sound awesome but how is it done? Any special equipment needed?
lotsofponies
Weanling
 
Posts: 36
Joined: Mon Jun 18, 2012 1:10 pm

Re: Single Layer Centrifugation (SLC)

Postby Jos » Fri Feb 01, 2013 1:44 pm

Our lab over in England worked with Jane Morell using Androcoll-E during the development of that product for use in "single layer centrifugation".

The concept works on the theory that damaged sperm will have a different density than non-damaged and this product Androcoll-E is in essence a one-step density gradient (rather than using other density gradients which can be made up using a cushion of different dilutions and therefore different densities) that will allow sperm of different densities to gradient at different points.

It showed value in removing damaged sperm in a normal or even low motility ejaculate, but unfortunately it was not beneficial in removing dead sperm from a stallion with spermiostasis (an "accumulator stallion"), in which situation the dead sperm layered along with the healthy sperm. It also showed some value for the removal of the EAV from shedding stallions ejaculates, although I would be a little nervous about the "absolute aspect" of that!!! :)

Jane's paper on the subject - along with links to other associated papers - can be found at: http://www.actavetscand.com/content/54/1/40
We're always happy to try and help, but don't forget to check the articles section
of the website too, which has a search engine to help you look for answers!
:)
User avatar
Jos
Senior Stallion or Mare
 
Posts: 3948
Joined: Sun Jun 10, 2012 12:11 am

Re: Single Layer Centrifugation (SLC)

Postby lotsofponies » Sat Feb 02, 2013 6:55 pm

Read the paper - WOW this is exciting... to be able to ship semen with Fedx on Friday and have usable semen on Monday. Thanks Jos
lotsofponies
Weanling
 
Posts: 36
Joined: Mon Jun 18, 2012 1:10 pm

Re: Single Layer Centrifugation (SLC)

Postby Jos » Sun Feb 03, 2013 5:29 am

lotsofponies wrote:...to be able to ship semen with Fedx on Friday and have usable semen on Monday.


I believe that aspect is going to be stallion-dependent to some extent. As a matter of routine, semen prepared correctly in the right extender for that stallion and centrifuged to remove seminal plasma will have greater longevity, without the use of Androcoll-E. One also has to bear in mind that if a sperm is still motile over an extended period of time, that does not necessarily correlate to being fertile. If capacitation has occurred some time previously during transit or storage, then the sperm may no longer be able to fertilize even though it is motile.
We're always happy to try and help, but don't forget to check the articles section
of the website too, which has a search engine to help you look for answers!
:)
User avatar
Jos
Senior Stallion or Mare
 
Posts: 3948
Joined: Sun Jun 10, 2012 12:11 am


Return to Breeding Methods

Who is online

Users browsing this forum: No registered users and 2 guests


Please note that opinions, product information, advice or suggestions posted on this bulletin board are not necessarily those of the management at Equine-Reproduction.com nor does the maintenance of the post position indicate an implicit or any endorsement of that information, opinion or product.

Further, although we have the greatest respect for the posters offering assistance here, you are advised to seek a consultation with your veterinarian prior to using information obtained from this board if it is of a veterinary nature.

cron



MAIN PAGE | INFORMATIONAL ARTICLES | SHORTCOURSES | INDIVIDUAL TUITION | SERVICES | LINKS
SEMEN EXPORT | STALLION STATION | FROZEN STALLIONS | FREEZING LOCATIONS | SUPPLIES | BOOKS
EQUINE REPRODUCTION E-MAIL LIST | SEMEN CALCULATOR | BULLETIN BOARD | CONTACT US