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Loss of semen

This category is to be used for general stallion questions that are not covered by other category descriptions.

Moderator: Jos

Loss of semen

Postby DawnYL6161 » Wed Jun 11, 2014 5:57 am

Need help please. We have been collecting a miniature stallion with great success until yesterday. Before: volume 10 ml, 432 count, 75% mobility, settling mares. Yesterday: 10 ml, 435 count, 0% mobility. All semen was dead. Handled all the same, diet not changed in stallion. In all the years we have been collecting this is a first. Any ideas?
DawnYL6161
Neonate
 
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Re: Loss of semen

Postby Jos » Wed Jun 11, 2014 8:07 am

In a situation like this, the first thing to look for is contamination. Are you using disposable AV liners (you should be!)? if not, is there something left behind in the washing process? Change the AV lubricant and try again in case that was contaminated. New everything...

If none of that proves to be the source of the problem, move to looking at the ejaculate. When was the stallion last collected prior to this problem collection? If it was a few weeks, there may be an issue with spermiostasis (where the stallion is not shedding sperm in his urine each day, which means you will need to collect him regularly to keep him cleaned out). Was there a lot of gel fraction in the problem ejaculation? If there was, gel contamination can kill sperm, so it needs to b filtered out effectively - sometimes one filter is not enough and two are required "in line". You can also fractionate the ejaculate to capture only the first three ejaculatory spurts, which contain most of the sperm. This avoids capturing the seminal plasma and the gel fraction.

Hope one of these items helps!
We're always happy to try and help, but don't forget to check the articles section
of the website too, which has a search engine to help you look for answers!
:)
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Re: Loss of semen

Postby DawnYL6161 » Mon Jun 16, 2014 6:42 pm

Ok finally time to reply.... Bought ALL new, liners, filters, lube, syringes, everything new but the actual CO AV. Same results. Spoke with local equine repo vet and he said probably he has a fever maybe even a 24 hour fever about 70 days back. We are to collect him once a week and see if they come back to life. Wish us luck...
DawnYL6161
Neonate
 
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Re: Loss of semen

Postby Jos » Mon Jun 16, 2014 8:20 pm

I have posted the following response to your same question on the EquineRepro Facebook Group:

Have you looked closely at the ejaculate as I outlined above? While an elevated temperature 57 days ago may be the issue, one would expect to see some earlier deterioration rather than a sudden drop - unless he has just been ill - as the sperm stored from the point of "manufacture" until ejaculation (i.e. 57 day's worth from the point of sickness on) would all be affected, and the temperature spike would be clearly identifiable if so recent (obviously 2 months ago, one might not notice or remember).

Has there been an increase in gel? If so, are you filtering in line, or is the ejaculate becoming contaminated by the gel? Gel fraction often increases in the height of the breeding season with some stallions, and can be highly spermicidal. Single filtration may not be enough - a double in-line filter may be needed.

Have you tried collecting the semen directly into warmed semen extender? Put about 25 ml of warmed extender into the collection bottle and then go and collect. Sometimes seminal plasma components can change during the breeding season, and toxicity can become more pronounced.

Finally, repeatedly collecting, rather than once a week might make a difference. If there is a spermiostasis situation, then cleaning him out by collecting 2 or 3 times a day for several days in a row might show an improvement, and be diagnostic in itself.
We're always happy to try and help, but don't forget to check the articles section
of the website too, which has a search engine to help you look for answers!
:)
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Jos
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Re: Loss of semen

Postby DawnYL6161 » Mon Jun 16, 2014 9:00 pm

No increase in gel....little if any gel associated in ejaculates.

Have not tried collection into warmed extender can do that.

Ruff to collect little 28" guy 2 to 3 times a day....time management thing but will tell her. Thanks for all the help.
DawnYL6161
Neonate
 
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Joined: Tue Apr 09, 2013 4:57 pm

Re: Loss of semen

Postby Chris » Wed Nov 12, 2014 5:58 pm

Is there a smaller CO AV available for collecting Mini's ?

As soon as I read the post I was thinking contamination issues as well as it happened to me once early in my collecting career (never since though)

Is there a possibility the thermometer you check your water temp with is faulty? If the lumen temp is too hot that will kill most of the sperm
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Location: Auckland, NEW ZEALAND

Re: Loss of semen

Postby Jos » Wed Nov 12, 2014 7:38 pm

There is indeed a short Colorado AV made for miniatures. Both 12" and 15" models are available. One can also use a Roanoke AV, although for many the INRA model AV will work - and there is even a miniature Missouri AV available through Har-Vet (although I'm not a big lover of the Missouri AV even though I used one for years...!). One of the issues with many Colorado models is that the glans of the penis remains in the lumen of the AV and sperm become heat-shocked owing to their passage over the heated lumen...
We're always happy to try and help, but don't forget to check the articles section
of the website too, which has a search engine to help you look for answers!
:)
User avatar
Jos
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Posts: 3948
Joined: Sun Jun 10, 2012 12:11 am


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